"Utilization of Electrochemical Sensors and Biosensors in Biochemistry and Molecular Biology" - Sensors Journal

Guest Editor
Dr. René Kizek
Department of Chemistry and Biochemistry
Mendel University of Agriculture and Forestry
Laboratory of Molecular Biochemistry and Bioelectrochemistry
Zemedelska 1, 613 00 Brno, Czech Republic
Tel. +420 5 45133350; Fax +420-545212044
E-mail: [email protected]; http://old.mendelu.cz/~kizek/


Summary

Biochemical and molecular-biological techniques still booms at all branches of biological research. Since successfully sequencing of human genome, rapid analysis of nucleic acids by various techniques has been developing intensively. The attention is mostly paid to recognizing of pathological sequence, which is related to diseases origin. Other field of research associated with nucleic acids analysis bases on studying and determining of changes of mRNA level as marker of various gene activity. “Switching of genes” is very common during development of all living beings, but the wrong timing of such switching can lead to many disorders and diseases including cancer. As it has been shown, count of human functional genes is very low, under 20 thousands (according to the last estimation), and thus can not be associated only with an organism development. Based on the mentioned facts, proteins are other group of biologically active compounds associated with the organism development. Research devoted to study of proteins called “proteomic research” is at very beginning.
Investigation the mentioned biological pathways can be done using battery techniques and methods. Very interesting and promising results bring techniques using various sensors and biosensors coupled with different types of detectors

This issue will include the following topics:

1. suggestion and construction of sensors and biosensors for molecular and biochemical applications;
2. new materials to construct of sensors (nanotechnology);
3. advantages and disadvantages of various types of detection of biologically important compounds;
4. sensors and biosensors for analysis of DNA and RNA;
5. sensors and biosensors for analysis of aminoacids, peptides and proteins;
6. chips and their utilizing for detection of DNA, RNA and proteins;
7. practical aspects of utilizing of sensors and biosensors in biology: estimation of specific sequence of animals, plants; monitoring of biomarkers; human and veterinary medicine (diagnostic of diseases).

Keywords

electrochemical sensors, DNA sensors, detection of proteins and DNA, molecular biology, cancer, human disease, sensor arrays, DNA and RNA chips, protein chips

Published Papers


Veronika Supalkova ^1,2, Jiri Petrek ¹, Jiri Baloun ^1,2, Vojtech Adam ^1,3, Karel Bartusek ⁴, Libuse Trnkova ⁵, Miroslava Beklova ⁶, Vaclav Diopan ^1,2, Ladislav Havel ¹ and René Kizek ^2,*
1 Department of Plant Biology, ² Department of Chemistry and Biochemistry, and ³ Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
⁴ Institute of Scientific Instruments, Academy of Sciences of the Czech Republic, Kralovopolska 147, CZ-612 00 Brno, Czech Republic
⁵ Department Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
⁶ Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
* Author to whom correspondence should be addressed; E-mail: [email protected]
Received: 5 May 2007 / Accepted: 23 May 2007 / Published: 31May 2007
Full Paper: Multi-instrumental Investigation of Affecting of Early Somatic Embryos of Spruce by Cadmium(II) and Lead(II) Ions
Sensors 2007, 7, 743-759 (PDF format, 515 K)


Veronika Supalkova 1,2, Dalibor Huska 1,2, Vaclav Diopan 1,2, Pavel Hanustiak 3, Ondrej Zitka 2,4, Karel Stejskal 2,4, Jiri Baloun 1,2, Jiri Pikula 3, Ladislav Havel 1, Josef Zehnalek 2, Vojtech Adam 2,5, Libuse Trnkova 6, Miroslava Beklova 3 and Rene Kizek 2*
¹ Department of Plant Biology, 2 Department of Chemistry and Biochemistry, and ⁵ Department of Animal Nutrition and Forage Production, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
³ Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
⁵ Department of Biochemistry, and 6 Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
* Author to whom correspondence should be addressed; E-mail: [email protected]
Received: 18 May 2007 / Accepted: 12 June 2007 / Published: 13 June 2007
Full Paper: Electroanalysis of Plant Thiols
Sensors 2007, 7, 932-959 (PDF format, 595 K)


Jaromir Hubalek ¹, Jan Hradecky ², Vojtech Adam ^2,3, Olga Krystofova ², Dalibor Huska ², Michal Masarik ⁴, Libuse Trnkova ⁵, Ales Horna ⁶, Katerina Klosova ¹, Martin Adamek ¹, Josef Zehnalek ² and Rene Kizek ^1,*
1 Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic
² Department of Chemistry and Biochemistry, and ³ Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
⁴ Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Komenskeho namesti 2, CZ-662 43 Brno, Czech Republic
⁵ Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
⁶ Department of Food Engineering, Faculty of Technology, Tomas Bata University, T.G. Masaryka 275, CZ-762 72 Zlin, Czech Republic
* Author to whom correspondence should be addressed; E-mail: [email protected]
Received: 3 July 2007 / Accepted: 13 July 2007 / Published: 16 July 2007
Full Paper: Spectrometric and Voltammetric Analysis of Urease – Nickel Nanoelectrode as an Electrochemical Sensor
Sensors 2007, 7, 1238-1255 (PDF format, 246 K)


Ondrej Zitka ^1,2, Dalibor Huska ¹, Sona Krizkova ¹, Vojtech Adam ^1,3, Grace J. Chavis ⁴, Libuse Trnkova ⁵, Ales Horna ⁶, Jaromir Hubalek ⁷ and Rene Kizek ^1,*
1 Department of Chemistry and Biochemistry, and ³ Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
² Department of Biochemistry, and ⁵ Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
⁴ Department of Chemistry, University of California, One Shields Avenue, CA-956 16 Davis, USA
⁶ Department of Food Engineering, Faculty of Technology, Tomas Bata University, T.G. Masaryka 275, CZ-762 72 Zlin, Czech Republic
⁷ Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic
* Author to whom correspondence should be addressed; E-mail: [email protected]
Received: 15 June 2007 / Accepted: 13 July 2007 / Published: 20 July 2007
Full Paper: An Investigation of Glutathione-Platinum(II) Interactions by Means of the Flow Injection Analysis Using Glassy Carbon Electrode
Sensors 2007, 7, 1256-1270 (PDF format, 124 K)


Sona Krizkova ¹, Miroslava Beklova ², Jiri Pikula ^2,*, Vojtech Adam ¹, Ales Horna ³ and René Kizek ^1
1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
² Department of Veterinary Ecology and Environmental Protection, Veterinary and Pharmaceutical University Brno, Palackeho 1/3, CZ-612 42 Brno, Czech Republic
³ Department of Food Engineering, Faculty of Technology, Tomas Bata University, T.G. Masaryka 275, CZ-762 72 Zlin, Czech Republic
* Author to whom correspondence should be addressed; E-mail:[email protected]
Received: 28 June 2007 / Accepted: 18 July 2007 / Published: 20 July 2007
Full Paper: Hazards of Secondary Bromadiolone Intoxications Evaluated using High-performance Liquid Chromatography with Electrochemical Detection
Sensors 2007, 7, 1271-1286 (PDF format, 276 K)


Vojtech Adam ^1,2, Radka Mikelova ³, Jaromír Hubalek ⁴, Pavel Hanustiak ⁵, Miroslava Beklova ⁵, Petr Hodek ⁶, Ales Horna ⁷, Libuse Trnkova ⁴, Marie Stiborova ⁶, Ladislav Zeman ², Rene Kizek ^1,*
1 Department of Chemistry and Biochemistry, and ² Department of Animal Nutrition and Forage Production Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic, *[email protected]
³ Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 37, CZ-611 37 Brno, Czech Republic
⁴ Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic
⁵ Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
⁶ Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, CZ-128 40 Prague, Czech Republic
⁷ Department of Food Engineering, Faculty of Technology, Tomas Bata University, CZ-762 72 Zlin, Czech Republic
* Author to whom correspondence should be addressed; e-mail:[email protected]
Received: 10 September 2007 / Accepted: 17 October 2007 / Published: 19 October 2007
Full Paper: Utilizing of Square Wave Voltammetry to Detect Flavonoids in the Presence of Human Urine
Sensors 2007, 7, 2402-2418 (PDF format, 573 K)


Vojtech Adam ^1,2, Miroslava Beklova ³, Jiri Pikula ³, Jaromir Hubalek ⁴, Libuse Trnkova ⁴ and Rene Kizek ^1,*
1 Department of Chemistry and Biochemistry, and ² Department of Animal Nutrition and Forage Production Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic, *[email protected]
³ Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
⁴ Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic
⁵ Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 37, CZ-611 37 Brno, Czech Republic
* Author to whom correspondence should be addressed; e-mail:[email protected]
Received: 1 October 2007 / Accepted: 17 October 2007 / Published: 19 October 2007
Full Paper: Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species
Sensors 2007, 7, 2419-2429 (PDF format, 235 K)


Marcela Havelková ^1,*, Tomáš Randák ², Vladimír Žlábek ², Jan Krijt ³, Hana Kroupová ², Jana Pulkrabová ⁴ and Zdeňka Svobodová ^1,2
1 University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Palackeho 1-3, 612 42 Brno, Czech Republic
2 University of South Bohemia Ceske Budejovice, Research Institute of Fish Culture and Hydrobiology Vodňany, Zatisi 728/II, 389 25 Vodňany, Czech Republic
3 Charles University, First Faculty of Medicine, Institute of Pathological Physiology, U Nemocnice 5, 128 53 Praha 2, Czech Republic
4 Institute of Chemical Technology Prague, Department of Food Chemistry and Analysis, Technicka 3, 166 28 Praha 6, Czech Republic
* Author to whom correspondence should be addressed. E-mail: [email protected]
Received: 18 September 2007 / Accepted: 1 November 2007 / Published: 2 November 2007
Full Paper: Biochemical Markers for Assessing Aquatic Contamination
Sensors 2007, 7, 2599-2611 (PDF format, 354 K)


Dun Pan, Xiaolei Zuo, Ying Wan, Lihua Wang, Jiong Zhang, Shiping Song^* and Chunhai Fan^*
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;
E-mails: {pandun, wanying, wanglihua, zj}@sinap.ac.cn, [email protected].
* Author to whom correspondence should be addressed; E-mails: {spsong, fchh}@sinap.ac.cn.
Received: 31 October 2007 / Accepted: 9 November 2007 / Published: 12 November 2007
Full Research Paper: Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue
Sensors 2007, 7, 2671-2680 (PDF format, 642 K)


Miroslav Pohanka ^1,*, František Treml ², Martin Hubálek ³, Hana Banďouchová ⁴, Miroslava Beklová ⁴ and Jiří Pikula ⁴
1 Center of Biological Defense, Těchonín, Czech Republic. E-mail: [email protected]
2 Department of Infectious Diseases and Epizootiology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. E-mail: [email protected]
3 Institute of Molecular Pathology, University of Defence, Hradec Králové, Czech Republic. E-mail: [email protected]
4 Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. E-mail: [email protected]
* Author to whom correspondence should be addressed. E-mail: [email protected]
Received: 17 October 2007 / Accepted: 14 November 2007 / Published: 19 November 2007
Full Research Paper: Piezoelectric Biosensor for a Simple Serological Diagnosis of Tularemia in Infected European Brown Hares (Lepus europaeus)
Sensors 2007, 7, 2825-2834 (PDF format, 271 K)


Matija Strlič ^1,*, Irena Kralj Cigić ², Jana Kolar ³, Gerrit de Bruin ⁴ and Boris Pihlar ²
1 University College London, Centre for Sustainable Heritage, The Bartlett School of Graduate Studies (Torrington Place site), Gower Street, London WCIE 6BT, UK,
E-mail: [email protected]
2 University of Ljubljana, Faculty of Chemistry and Chemical Technology, Ljubljana, Slovenia
E-mail:[email protected]; [email protected]
3 National and University Library, Ljubljana, Slovenia, E-mail: [email protected]
4 Nationaal Archief, The Hague, The Netherlands, E-mail: [email protected]
* Author to whom correspondence should be addressed.
Received: 12 November 2007 / Accepted: 4 December 2007 / Published: 5 December 2007
Full Research Paper: Non-Destructive Evaluation of Historical Paper Based on pH Estimation from VOC Emissions
Sensors 2007, 7, 3136-3145 (PDF format, 527 K)


Jianqiang Hu, Zhouping Wang and Jinghong Li*
Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China
E-mail: [email protected]
Received: 12 November 2007 / Accepted: 12 December 2007 / Published: 14 December 2007
Full Research Paper: Gold Nanoparticles With Special Shapes: Controlled Synthesis, Surface-enhanced Raman Scattering, and The Application in Biodetection
Sensors 2007, 7, 3299-3311 (PDF format, 1470 K)


Mun'delanji Vestergaard ¹, Kagan Kerman ² and Eiichi Tamiya ^3,*
1 School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST),1-1 Asahidai, Nomi City, Ishikawa 923-1292, Japan
2 Dept. of Chemistry, University of Western Ontario, 1151 Richmond Street, London, Ontario, N6A 5B7, Canada
3 Nanobiotechnology and Biodevice Laboratory, Dept. of Applied Physics, Graduate School of Engineering, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047
* Author to whom correspondence should be addressed. E-mail: [email protected]
Received: 4 December 2007 / Accepted: 18 December 2007 / Published: 20 December 2007
Review: Label-free Electrochemical Protein Sensors
Sensors 2007, 7, 3442-3458 (PDF format, 496 K)


Petra Horaková-Brazdilova ^1,2, Miloslava Fojtova ¹, Karel Vytras ² and Miroslav Fojta ^1,*
1 Institute of Biophysics, v.v.i., Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-612 65 Brno, Czech Republic. E-mail: [email protected], [email protected], [email protected]
2 Department of Analytical Chemistry, University of Pardubice, Nam. Cs. Legii 565, CZ-53210, Czech Republic. E-mail: [email protected]
* Author to whom correspondence should be addressed.
Received: 28 December 2007 / Accepted: 7 January 2008 / Published: 10 January 2008
Full Research Paper: Enzyme-Linked Electrochemical Detection of PCR-amplified Nucleotide Sequences Using Disposable Screen-Printed Sensors. Applications in Gene Expression Monitoring
Sensors 2008, 8, 193-210 (PDF format, 448 K)


Umasankar Yogeswaran and Shen-Ming Chen * 
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road , Taipei 106, Taiwan (ROC).
* Fax: +886 2270 25238; Tel: +886 2270 17147; E-mail: [email protected]
Received: 29 December 2007 / Accepted: 14 January 2008 / Published: 21 January 2008
Review: A Review on the Electrochemical Sensors and Biosensors Composed of Nanowires as Sensing Material
Sensors 2008, 8, 290-313 (PDF format, 745 K)


Libuse Trnkova ^1,*, Lenka Zerzankova ¹, Filip Dycka ¹, Radka Mikelova ¹ and Frantisek Jelen ²
1 Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic
2 Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, Brno 612 65, Czech Republic
* Author to whom correspondence should be addressed; E-mail:[email protected]
Received: 30 December 2007 / Accepted: 15 January 2008 / Published: 23 January 2008
Full Paper: Study of Copper and Purine-Copper Complexes on Modified Carbon Electrodes by Cyclic and Elimination Voltammetry
Sensors 2008, 8, 429-444 (PDF format, 515 K)


Sona Krizkova ¹, Pavel Ryant ², Olga Krystofova ³, Vojtech Adam ^1,4, Michaela Galiova ³, Miroslava Beklova ⁵, Petr Babula ⁶, Jozef Kaiser ⁷, Karel Novotny ³,
Jan Novotny ⁷, Miroslav Liska ⁷, Radomir Malina ⁷, Josef Zehnalek ¹, Jaromir Hubalek ⁸, Ladislav Havel ⁹ and Rene Kizek ^1,*
1 Department of Chemistry and Biochemistry, 2 Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, 4 Department of Animal Nutrition and Forage Production, and 9 Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
3 Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic
5 Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, and 6 Department of Natural Drugs, Faculty of Pharmacy and University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
7 Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, Technicka 2896/2, CZ-616 69 Brno, Czech Republic
8 Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic
* Author to whom correspondence should be addressed; e-mail:[email protected]
Received: 26 December 2007 / Accepted: 15 January 2008 / Published: 24 January 2008
Full Paper: Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions – Plants as Bioindicators of Environmental Pollution
Sensors 2008, 8, 445-463 (PDF format, 1270 K)


Vojtech Adam ^1,2, Ondrej Zitka ¹, Petr Dolezal ², Ladislav Zeman ², Ales Horna ³, Jaromir Hubalek ⁴, Jan Sileny ⁴, Sona Krizkova ¹, Libuse Trnkova ⁵ and Rene Kizek ^1,*
1 Department of Chemistry and Biochemistry, and 2 Department of Animal Nutrition and Forage Production Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
3 Department of Food Engineering, Faculty of Technology, Tomas Bata University, T.G. Masaryka 275, CZ-762 72 Zlin, Czech Republic
4 Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic
5 Department Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
* Author to whom correspondence should be addressed; e-mail:[email protected]
Received: 27 December 2007 / Accepted: 15 January 2008 / Published: 24 January 2008
Full Paper: Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector
Sensors 2008, 8, 464-487 (PDF format, 592 K)


S. Ashok Kumar and Shen-Ming Chen *
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, section 3, Chung-Hsiao East Road, Taipei, 106, Taiwan (ROC)
* Author to whom correspondence should be addressed. Fax: +886 2270 25238; Tel: +886 2270 17147.
E-mail addresses: [email protected], [email protected]
Received: 31 December 2007 / Accepted: 30 January 2008 / Published:  6 February 2008
Review: Electroanalysis of NADH Using Conducting and Redox Active Polymer/Carbon Nanotubes Modified Electrodes-A Review
Sensors 2008, 8, 739-766 (PDF format, 500 K)


Dalibor Huska ^1,2, Sona Krizkova ¹, Miroslava Beklova ³, Ladislav Havel ², Josef Zehnalek ¹, Vaclav Diopan ^1,2, Vojtech Adam ^1,4, Ladislav Zeman ⁴,
Petr Babula ⁵ and Rene Kizek ^1,*
1Department of Chemistry and Biochemistry, 2Department of Plant Biology, and 4Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
3Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, and 5Department of Natural Drugs, Faculty of Pharmacy and University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
* Author to whom correspondence should be addressed; E-mail:[email protected]
Received: 31 January 2008 / Accepted: 14 February 2008 / Published: 19 February 2008
Full Paper: Influence of Cadmium(II) Ions and Brewery Sludge on Metallothionein Level in Earthworms (Eisenia fetida) – Bio-transforming of Toxic Wastes
Sensors 2008, 8, 1039-1047 (PDF format, 267 K)


Wataru Satoh, Hiroki Hosono, Hiroomi Yokomaku, Katsuya Morimoto, Sanjay Upadhyay and Hiroaki Suzuki *
Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan, Phone: +81-29-853-5598, Fax: +81-29-853-4490;
E-mails: [email protected], [email protected], [email protected], [email protected], s [email protected]
* Author to whom correspondence should be addressed. E-mail: [email protected]
Received: 17 January 2008 / Accepted: 18 February 2008 / Published: 21 February 2008
Full Research Paper: Integrated Electrochemical Analysis System with Microfluidic and Sensing Functions
Sensors 2008, 8, 1111-1127 (PDF format, 1310 K)


Sungbo Cho ^1,*, Marc Castellarnau ^2,3,*, Josep Samitier ^2,3 and Hagen Thielecke ¹
1 Biohybrid Systems, Fraunhofer Institute for Biomedical Engineering, Ensheimer Str. 48 66386 St. Ingbert Germany
2 Nanobioengineering Research Laboratory, Bioengineering Institute of Catalonia, Barcelona Science Park, Josep Samitier 1-5, 08028 Barcelona, Spain
3 Electronics Department, University of Barcelona, Martí i Franquès 1, Planta 2 08028 Barcelona, Spain
* E-mail: [email protected], [email protected]; contributed equally.
Received: 31 January 2008 / Accepted: 20 February 2008 / Published: 22 February 2008
Full Research Paper: Dependence of Impedance of Embedded Single Cells on Cellular Behaviour
Sensors 2008, 8, 1198-1211 (PDF format, 1431 K)


Ee Taek Hwang ¹, Joo-Myung Ahn ¹, Byoung Chan Kim ² and Man Bock Gu ^1,*
1 College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-Gu, Seoul, Republic of Korea
2 Diagnostics Group, Institut Pasteur Korea, Hawolgok-dong, Seongbuk-gu, Seoul, Republic of Korea
* Author to whom correspondence should be addressed; [email protected]
Received: 31 January 2008 / Accepted: 21 February 2008 / Published: 22 February 2008
Full Research Paper: Construction of a nrdA::luxCDABE Fusion and Its Use in Escherichia coli as a DNA Damage Biosensor
Sensors 2008, 8, 1297-1307 (PDF format, 252 K)


María Soledad Belluzo, María Élida Ribone and Claudia Marina Lagier *
Analytical Chemistry Department, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario –2000- Argentina
E-mail: [email protected]
* Author to whom correspondence should be addressed.
Received: 1 February 2008 / Accepted: 14 February 2008 / Published: 27 February 2008
Review: Assembling Amperometric Biosensors for Clinical Diagnostics
Sensors 2008, 8, 1366-1399 (PDF format, 564 K)


Dorothee Grieshaber ¹, Robert MacKenzie ¹, Janos Vörös ¹ and Erik Reimhult ^2,*
1 Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Gloriastrasse 35, 8092 Zurich, Switzerland
E-mail: [email protected]. E-mail: [email protected]. E-mail: [email protected]
2 Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, Wolfgang-Pauli-Strasse 10, 8093 Zurich, Switzerland
E-mail: [email protected]
* Author to whom correspondence should be addressed.
Received: 24 January 2008 / Accepted: 28 January 2008 / Published: 3 March 2008
Review: Electrochemical Biosensors - Sensor Principles and Architectures
Sensors 2008, 8, 1400-1458 (PDF format,  K)


Adina Arvinte, Lucian Rotariu and Camelia Bala *
Laboratory of Quality Control and Process Monitoring, Department of Analytical  Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Romania
* Author to whom correspondence should be addressed. E-mail: [email protected]
Received: 4 February 2008 / Accepted: 21 February 2008 / Published: 3 March 2008
Full Research Paper: Amperometric Low-potential Detection of Malic Acid Using Single-wall Carbon Nanotubes Based Electrodes
Sensors 2008, 8, 1497-1507 (PDF format, 216 K)


Fabiane C. de Abreu ^1,*, Francine S. de Paula ¹, Danielle C. M. Ferreira ¹, Valberes B. Nascimento ², Julio C. D. Lopes ^3,*, Alexandre M. C. Santos ⁴, Marcelo M. Santoro ⁴, Carlos E. Salas ⁴ and Marília O. F. Goulart ^1,*
1 Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió, AL, Brazil. E-mail: [email protected]. E-mail: [email protected]. E-mail: [email protected]
2 Departamento de Química, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil. E-mail: [email protected]
3 Departamento de Química, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. E-mail: [email protected]
4 Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. E-mail: [email protected]. E-mail: [email protected].
E-mail: [email protected]
* Author to whom correspondence should be addressed. Dr. Marília Oliveira Fonseca Goulart; Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Campus A.C. Simões, Tabuleiro do Martins, 57072-970, Maceió, AL, Brazil; Tel./Fax: + 55-82-32141389; E-mail address: [email protected]
Received: 29 January 2008 / Accepted: 14 February 2008 / Published: 3 March 2008
Full Research Paper: The Application of DNA-biosensors and Differential Scanning Calorimetry to the Study of the DNA-binding Agent Berenil
Sensors 2008, 8, 1519-1538 (PDF format,  K)


Fei Huang,  Youyuan Peng,  Guiying Jin,  Song Zhang and Jilie Kong*
Department of Chemistry, Fudan University, Shanghai 200433, P.R. China
*Author to whom correspondence should be addressed; E-mail: [email protected]
Received: 1 February 2008 / Accepted: 14 March 2008 / Published: 17 March 2008
Full Research Paper: Sensitive Detection of Haloperidol and Hydroxyzine at Multi-Walled Carbon Nanotubes Modified Glassy Carbon Electrode
Sensors 2008, 8, 1879-1889 (PDF format, 88 K)


Liang Ming ¹, Xia Xi ¹, Tingting Chen ¹ and Jie Liu ^2,*
1 The School of Chemistry and Chemical Technology, Nantong University, Nantong, Jiangsu 226001, P.R. China; E-mails: [email protected] (LM); [email protected] (XX); [email protected] (TC)
2 Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, P.R. China
*Author to whom correspondence should be addressed; E-mail: [email protected]
Received: 23 January 2008 / Accepted: 20 February 2008 / Published:  17 March 2008
Full Research Paper: Electrochemical Determination of Trace Sudan I Contamination in Chilli Powder at Carbon Nanotube Modified Electrodes
Sensors 2008, 8, 1890-1900 (PDF format, 371 K)


You Wang, Hui Xu, Jianming Zhang and Guang Li *
State Key Laboratory of Industrial Control Technology, Institute of Advanced Process Control, Zhejiang University, Hangzhou 310027, P.R. China; E-mails: [email protected] (You Wang); [email protected] (Hui Xu); [email protected] (Jianming Zhang)
* Author to whom correspondence should be addressed; E-mail: [email protected]; Tel: +86-13558068126
Received: 31 January 2008 / Accepted: 4 March 2008 / Published: 27 March 2008
Review: A Review of Electrochemical Sensors for Clinic Analysis
Sensors 2008, 8, 2043-2081 (PDF format, 367 K)


Vojtech Adam ^1,2, Jiri Baloun ¹, Ivo Fabrik ^1,3, Libuse Trnkova ⁴ and Rene Kizek ^1,*
1 Department of Chemistry and Biochemistry and 2 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic; E-mail: [email protected]
3 Department of Chemistry and  4 Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
* Author to whom correspondence should be addressed; E-mail: [email protected]
Received: 15 March 2008 / Accepted: 26 March 2008  / Published: 1 April 2008
Full Paper: An Electrochemical Detection of Metallothioneins in Nanolitres at Zeptomole Level
Sensors 2008, 8, 2293-2305 (PDF format, 151 K)


Marcela Havelkova ^1,*, Jana Blahova ¹, Hana Kroupova ², Tomas Randak ², Iveta Slatinska ¹, Drahomira Leontovycova ³, Roman Grabic ⁴, Richard Pospisil ⁵ and Zdenka Svobodova ^1,2
1 University of Veterinary and Pharmaceutical Sciences Brno, Department of Veterinary Public Health and Toxicology, Palackeho 1-3, 612 42 Brno, Czech Republic; E-mail: [email protected]
2 University of South Bohemia Ceske Budejovice, Research Institute of Fish Culture and Hydrobiology Vodnany, Zatisi 728/II, 389 25 Vodnany, Czech Republic
3 Czech Hydrometeorological Institute, Na Sabatce 17, 143 06 Praha 4, Czech Republic
4 Institute of Public Health Ostrava, Partyzanske nam. 7, 702 00 Ostrava, Czech Republic
5 Palacky University Olomouc, Faculty of Law, Department of Financial Law and Economics, Tr. 17. listopadu 8, 771 00 Olomouc, Czech Republic
* Author to whom correspondence should be addressed; E-mail: [email protected]
Received: 31 March 2008 / Accepted: 10 April 2008 / Published: 11 April 2008
Full Research Paper: Biomarkers of contaminant exposure in Chub (Leuciscus cephalus L.) – a biomonitoring of major rivers in the Czech Republic
Sensors 2008, 8, 2589-2603 (PDF format, 625 K)


Sophie Mavrikou ^1,2, Kelly Flampouri ^1,2, Georgia Moschopoulou ^1,2, Olga Mangana ³, Alexandros Michaelides ² and Spiridon Kintzios ^1,2,*
1    Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural University of Athens, Greece
2    EMBIO Diagnostics Project, Nicosia, Cyprus
3    Ministry of Agriculture, Centre of Athens Veterinary Institutions, Institute of Infectious and Parasitic Diseases, Virus Laboratory, Athens, Greece
*Author to whom correspondence should be addressed; Tel. +30 210 529 429; Fax. +30 210 529 4286; E-Mail: [email protected]
Received: 29 January 2008 / Accepted: 22 April 2008 / Published: 23 April 2008
Full Research Paper: Assessment of Organophosphate and Carbamate Pesticide Residues in Cigarette Tobacco with a Novel Cell Biosensor
Sensors 2008, 8, 2818-2832 (PDF format, 422 K)


Sona Krizkova ¹, Ivo Fabrik ¹, Vojtech Adam ^1,2, Jiri Kukacka ³, Richard Prusa ³, Grace J. Chavis ⁴, Libuse Trnkova ⁵, Jan Strnadel ⁶, Vratislav Horak ⁶ and Rene Kizek ^1,*
1 Department of Chemistry and Biochemistry, and 2 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
3 Department of Clinical Biochemistry and Pathobiochemistry, 2nd Faculty of Medicine, Charles University, V Uvalu 84, CZ-150 06 Prague 5, Czech Republic
4 Department of Chemistry, University of California, One Shields Avenue, CA-956 16 Davis, USA
5 Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
6 Laboratory of Tumour Biology, Department of Animal Embryology, Cell and Tissue Differentitation, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., CZ-277 21 Libechov, Czech Republic
* Author to whom correspondence should be addressed; E-mail:kizek (a) sci.muni.cz
Received: 22 April 2008 / Accepted: 9 May 2008 / Published: 10 May 2008
Article: Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues
Sensors 2008, 8, 3106-3122 (PDF format, 185 K) DOI: 10.3390/s8053106


Pavel Ryant ¹, Eva Dolezelova ¹, Ivo Fabrik ², Jiri Baloun ², Vojtech Adam ^2,3, Petr Babula ⁴ and Rene Kizek ^2,*
1 Depatment of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, 2 Department of Chemistry and Biochemistry and 3 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
4 Deparment of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
* Author to whom correspondence should be addressed; E-mail:kizek (a) sci.muni.cz
Received: 29 April 2008 / Accepted: 14 May 2008 / Published: 15 May 2008
Article: Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)
Sensors 2008, 8, 3165-3182 (PDF format, 273 K) DOI: 10.3390/s8053165


Patricia A. Broderick ^1,2,3,*, Helen Ho ¹, Karyn Wat ¹ and Vivek Murthy ¹
1 Dept.of Physiology and Pharmacology, The City University of New York (CUNY) Medical
School, Sophie Davis School of Biomedical Education, City College of New York, NY, NY, USA
2 Doctoral Program, Dept. Biology, Dept. Psychology, CUNY Graduate School, NY, NY, USA
3 Dept.of Neurology, NYU School of Medicine, NYU Comprehensive Epilepsy Center, NY,NY,USA
* Author to whom correspondence should be addressed; E-mails: [email protected]; [email protected]; http://www.broderickprobe.com; Tel.: +1-212-650-5479;
Fax +1-212-650-7305
Received: 29 May 2008; in revised form: 30 June 2008 / Accepted: 2 July 2008 / Published: 4 July 2008
Article: Laurate Biosensors Image Brain Neurotransmitters In Vivo: Can an Antihypertensive Medication Alter Psychostimulant Behavior?
Sensors 2008, 8, 4033-4061 (PDF format, 540 K) DOI: 10.3390/s8074033


Ivo Fabrik ¹, Zuzana Ruferova ², Klara Hilscherova ², Vojtech Adam ^1,3, Libuse Trnkova ⁴ and Rene Kizek ^1,*
1 Department of Chemistry and Biochemistry, and 3 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
2 Research Centre for Environmental Chemistry and Ecotoxicology, and 4 Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
* Author to whom correspondence should be addressed; E-mail:[email protected]
Received: 31 May 2008; in revised form: 6 July 2008 / Accepted: 6 July 2008 / Published: 10 July 2008
Article: A Determination of Metallothionein in Larvae of Freshwater Midges (Chironomus riparius) Using Brdicka Reaction
Sensors 2008, 8, 4081-4094 (PDF format, 181 K) DOI: 10.3390/s8074081


Marcela Havelková ^1,*, Ladislav Dušek ², Danka Némethová ², Gorzyslaw Poleszczuk ³ and Zdeňka Svobodová ^1,4
1 University of Veterinary and Pharmaceutical Sciences, Faculty of Veterinary Hygiene and Ecology, Department of Public Health and Toxicology, Palackého 1-3, 612 42, Brno, Czech Republic
2 Masaryk University, Faculty of Medicine and Faculty of Science, Institute of Biostatistics and Analyses, Kamenice 126/3, 625 00 Brno, Czech Republic
3 Szczecin University, Faculty of Natural Sciences, Chair of Chemistry, ul. Felczaka 3A, 71-412 Szczecin, Poland
4 University of South Bohemia in České Budějovice, Research Institute of Fish Culture and Hydrobiology, 389 25 Vodňany, Czech Republic
* Author to whom correspondence should be addressed; E-mail: [email protected]
Received: 19 June 2008; in revised form: 6 July 2008 / Accepted: 6 July 2008 / Published: 10 July 2008
Article: Comparison of Mercury Distribution Between Liver and Muscle – A Biomonitoring of Fish from Lightly and Heavily Contaminated Localities
Sensors 2008, 8, 4095-4109 (PDF format, 409 K) DOI: 10.3390/s8074095

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MDPI - Matthias Burkhalter - 16 July 2008